Uni-Sine
UK examsA-level

AQA A-level Biology (7402): papers, topics and grade boundaries

Papers reset in

AQA A-level Biology papers at a glance

PaperTopicsLengthMarksShare of A-levelQuestion types
Paper 11 to 4 and relevant practical skills2 hours9135%76 marks short and long answer; 15 marks extended response
Paper 25 to 8 and relevant practical skills2 hours9135%76 marks short and long answer; 15 marks comprehension
Paper 31 to 8 and relevant practical skills2 hours7830%38 marks structured, including practical techniques; 15 marks critical analysis of data; 25 marks one essay from a choice of two

The qualification is linear, so all A-level exams are sat at the end of the course. AQA's June 2023 paper cover lists a scientific calculator. At least 10% of the marks across the assessments require mathematical skills at Level 2 or above.

The eight AQA A-level Biology topics

TopicTitleTested in
1Biological moleculesPapers 1 and 3
2CellsPapers 1 and 3
3Organisms exchange substances with their environmentPapers 1 and 3
4Genetic information, variation and relationships between organismsPapers 1 and 3
5Energy transfers in and between organisms (A-level only)Papers 2 and 3
6Organisms respond to changes in their internal and external environments (A-level only)Papers 2 and 3
7Genetics, populations, evolution and ecosystems (A-level only)Papers 2 and 3
8The control of gene expression (A-level only)Papers 2 and 3

Paper 3 has no topic list of its own: it can draw on topics 1 to 8 together, which is why synoptic revision matters. Topics 1 to 4 are the AS content too.

Understanding Paper 3 and the essay

Paper 3 is the most distinctive paper. It has 38 marks of structured questions that include practical techniques, 15 marks of critical analysis of given experimental data, and one essay from a choice of two titles, worth 25 marks. The essay rewards a planned answer that draws on several topics and uses correct terminology. A reliable approach is a short plan of 3 or 4 headings, an opening definition, then each paragraph making one point with evidence.

Practical endorsement and required practicals

A-level grades are based only on the written exams. Separately your teacher assesses your practical competence against the Common Practical Assessment Criteria (CPAC) across a minimum of 12 practical activities, and the result is reported as a pass or not classified. AQA's specification has 12 required practicals, and exam questions on practical skills and data analysis can use any of them. Analyse your own results in the Practical Data Lab.

Worked examples in AQA style

Populations: the Hardy-Weinberg equation

A recessive condition affects 1 in 1600 people. Treat the population as being in Hardy-Weinberg equilibrium. The frequency of the recessive allele q comes from q² = 1/1600:

q=\sqrt{\frac{1}{1600}}=0.025,\quad p=1-q=0.975,\quad 2pq=2\times0.975\times0.025=0.04875

About 4.9% of people are carriers. Check the arithmetic in the Hardy-Weinberg Calculator.

Cells: magnification

A mitochondrion is 2.4 μm long and its image is 36 mm long. Convert 36 mm to 36 000 μm first:

\text{magnification}=\frac{36000}{2.4}=15000

Statistics: a chi-squared test

A dihybrid cross produces 160 offspring. Expected 9 : 3 : 3 : 1 gives 90, 30, 30 and 10, and the observed numbers are 85, 33, 29 and 13. Then:

\chi^2=\sum\frac{(O-E)^2}{E}=\frac{25}{90}+\frac{9}{30}+\frac{1}{30}+\frac{9}{10}=1.51

There are 4 − 1 = 3 degrees of freedom. The critical value at the 0.05 probability level for 3 degrees of freedom is 7.815, so 1.51 is far below it. The difference between observed and expected is not significant, and the ratio fits. Practise predicting the odds in our Genetics and DNA course, then press Mark to check your answer in the course:

Type the chance that a child of a random cross has a given trait.

Press Mark to have your answer marked in the Genetics & DNA course.

AQA A-level Biology grade boundaries

These are AQA's subject grade boundaries from its document "Grade boundaries, June 2026 exams: A-level", published on 13 August 2026. Each figure is the lowest total mark, out of 260, that earns that grade.

June 2026A*ABCDE
Biology 7402, out of 2601991741461189063

An A* needed 199 of 260, which is 77%, and an A needed 174. An E needed 63, about a quarter of the marks.

How AQA A-level Biology differs from Edexcel

Pearson Edexcel offers two A level Biology specifications, Biology A (Salters-Nuffield, 9BN0) and Biology B (9BI0). Biology A has three 2-hour, 100-mark papers worth a third each, with a pre-released scientific article in Paper 3 and 18 core practicals. Biology B is 1 hour 45 minutes (90 marks), 1 hour 45 minutes (90 marks) and 2 hours 30 minutes (120 marks), worth 30%, 30% and 40%, with 16 core practicals. AQA's essay in Paper 3 is its own feature. See theEdexcel A-level Biology guide.

Use the daily paper as a short timed mock

Uni-Sine publishes a free daily paper on theA-level Biology: Paper 1andA-level Biology: Paper 2tracks. Each is a short timed paper of 8 questions worth 25 to 35 marks, not a full AQA paper, and it is not written by AQA. Track 1 covers biological molecules, cell structure, membranes, nucleic acids, enzymes, gas exchange, mitosis, immunity, transport and genetic diversity, which matches AQA's topics 1 to 4. Track 2 covers respiration, photosynthesis, energy in ecosystems, inheritance and Hardy-Weinberg, nervous coordination, muscle contraction, homeostasis, evolution and gene expression, matching topics 5 to 8. Because the papers are short, use the paper-style essay practice separately.

Common mistakes in AQA A-level Biology

  • Not planning the essay. A list of facts without a line of argument earns few marks.
  • Chi-squared with percentages. Use the actual counts, not percentages.
  • Confusing "recessive allele frequency" and "recessive genotype". q is the allele, q² is the genotype.
  • Ignoring the context. Quote data from the question when you describe a trend.

Exam answers

The answers and a worked mark scheme come with today's paper once it is marked. We do not publish official or past paper answers. Every question on the daily paper is our own.

Common questions

What is the AQA A-level Biology code?
The A-level specification code is 7402. The AS is 7401.
How many papers is AQA A-level Biology?
Three, each 2 hours. Paper 1 and Paper 2 are 91 marks (35% each) and Paper 3 is 78 marks (30%).
What is the AQA A-level Biology essay?
Paper 3 includes one essay from a choice of two titles, worth 25 marks.
How many required practicals are there in AQA A-level Biology?
12, assessed by your teacher for the practical endorsement and also tested in the written papers.
What were the AQA A-level Biology grade boundaries in June 2026?
Out of 260: A* needed 199, A needed 174, B needed 146, C needed 118, D needed 90 and E needed 63.

Revision cards

Say the answer first, then flip the card to check it.

Where to go next

  • Solve it

    Hardy-Weinberg Calculator

    Find allele and genotype frequencies in a population

    Open the tool
  • Learn it

    Genetics & DNA

    From Mendel to CRISPR: how traits are inherited and genomes are edited

    Start the course
  • Test it

    A-level Biology: Year 1

    A new exam-style paper every day, with instant marking and a mark scheme

    Start today's paper
Read next
UK · A-levelAQA A-level Biology grade boundaries 2026: A* to EAQA's official June 2026 A-level Biology (7402) grade boundaries, A* to E out of 260, compared with 2025, with a worked example.