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A-level Biology 2027: AQA, OCR and Edexcel papers, exam dates and grade boundaries

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A-level Biology exam boards and specifications

Your school chooses the board and the specification, so ask your teacher which one you are on before you buy a revision guide or download papers. There are five in England:

Exam boardSpecificationCodeTotal marks
AQABiology7402260
OCRBiology AH420270
OCRBiology B (Advancing Biology)H422270
EdexcelBiology A (Salters-Nuffield)9BN0300
EdexcelBiology B9BI0300

WJEC A-level Biology is the Welsh board's version and has its own structure. The rest of this guide covers the three English boards.

AQA A-level Biology specification

The AQA A-level Biology specification has eight topics. Topics 1 to 4 are shared with the AS, and topics 5 to 8 are A-level only.

  1. Biological molecules
  2. Cells
  3. Organisms exchange substances with their environment
  4. Genetic information, variation and relationships between organisms
  5. Energy transfers in and between organisms
  6. Organisms respond to changes in their internal and external environments
  7. Genetics, populations, evolution and ecosystems
  8. The control of gene expression
AQA paperTopicsTimeMarksShareWhat is in it
Paper 11 to 42 hours9135%Short and long answers, plus 15 marks of extended response
Paper 25 to 82 hours9135%Short and long answers, plus 15 marks of comprehension
Paper 31 to 82 hours7830%Structured questions, 15 marks of data analysis and a 25 mark essay

The essay is the big difference from other boards: you choose one of two titles, and it is worth 25 marks, nearly a third of Paper 3. Every paper also tests practical skills.

OCR A-level Biology specification

The OCR A-level Biology A specification is in six modules: development of practical skills, foundations in biology, exchange and transport, biodiversity, evolution and disease, communication, homeostasis and energy, and genetics, evolution and ecosystems.

OCR Biology A paperModulesTimeMarksShare
Biological processes (01)1, 2, 3 and 52 hours 15 minutes10037%
Biological diversity (02)1, 2, 4 and 62 hours 15 minutes10037%
Unified biology (03)All six1 hour 30 minutes7026%

OCR Biology B (Advancing Biology) uses the same timings with different papers: Fundamentals of biology (110 marks), Scientific literacy in biology (100 marks) and Practical skills in biology (60 marks).

Edexcel A-level Biology A and B

EdexcelPaper 1Paper 2Paper 3
Biology A (Salters-Nuffield)The Natural Environment and Species Survival: 2 hours, 100 marksEnergy, Exercise and Co-ordination: 2 hours, 100 marksGeneral and Practical Applications in Biology: 2 hours, 100 marks
Biology BAdvanced Biochemistry, Microbiology and Genetics: 1 hour 45 minutes, 90 marksAdvanced Physiology, Evolution and Ecology: 1 hour 45 minutes, 90 marksGeneral and Practical Principles in Biology: 2 hours 30 minutes, 120 marks

A-level Biology exam dates 2027

All three boards have published final timetables (AQA, OCR and Edexcel), and the biology papers fall on the same three days.

DateAQAOCR AOCR BEdexcel A and B
Monday 7 June 2027, afternoonPaper 1Biological processesFundamentals of biologyPaper 1
Monday 14 June 2027, afternoonPaper 2Biological diversityScientific literacy in biologyPaper 2
Friday 18 June 2027, morningPaper 3Unified biologyPractical skills in biologyPaper 3
  • Results day: Thursday 12 August 2027.
  • Contingency day: Wednesday 23 June 2027, in case of serious national disruption.
  • Entries: schools must enter you by 21 February 2027 (JCQ key dates).

A-level Biology grade boundaries

A grade boundary is the lowest total mark across all three papers that earns a grade. The boards set new boundaries every summer to allow for how hard the papers were. These are the latest, from June 2026 (AQA, OCR and Edexcel).

June 2026Out ofA*ABCDE
AQA 74022601991741461189063
OCR Biology A2702041761501249872
OCR Biology B27019317315313411596
Edexcel Biology A30022019617014411994
Edexcel Biology B30021818916013110273

AQA A-level Biology grade boundaries as percentages

In June 2026, AQA's A* took 199 out of 260 (76.5%), an A took 174 (66.9%), a C took 118 (45.4%) and an E took 63 (24.2%). The other boards were close: an A was 65.2% on OCR Biology A, 65.3% on Edexcel Biology A and 63.0% on Edexcel Biology B. Treat any boundary as a guide, because the 2027 figures will be different.

Required practicals and the Practical Endorsement

The Department for Education's rules say every A-level Biology student must carry out at least 12 practical activities, which count towards the Practical Endorsement. AQA calls them required practicals, OCR groups them into practical activity groups (PAGs), and Edexcel calls them core practicals.

  • The endorsement is separate. Your teacher assesses it, and it is reported alongside your grade without adding to it.
  • The written papers test practicals too. Expect questions on methods, variables, results tables, graphs and errors, set in the context of the practicals you did and ones you didn't.
  • Keep your lab book tidy. It is the evidence for your endorsement, and the best revision notes for the practical questions.

To practise analysing results, enter a data set in the Practical Data Lab and work out means, ranges and uncertainties.

Maths in A-level Biology, worked through

At least 10% of the marks test maths at GCSE (level 2) standard or above. The same few calculations come up again and again. We wrote the questions below for this guide.

Magnification

Question: a cell is 50 µm long, and its image in a textbook is 25 mm long. What is the magnification?

\text{magnification} = \frac{\text{image size}}{\text{actual size}} = \frac{25\ \text{mm}}{0.05\ \text{mm}} = 500

The answer is ×500. Put both sizes in the same unit first: 50 µm is 0.05 mm, because 1 mm is 1,000 µm. Mixing units is the commonest way to lose this mark. Check more in the Magnification Calculator.

Surface area to volume ratio

A cube with 2 cm sides has a surface area of 6 × 2 × 2 = 24 cm² and a volume of 8 cm³, a ratio of 3:1. Double the side to 4 cm and you get 96 cm² over 64 cm³, only 1.5:1. That halving is why large organisms need exchange surfaces and transport systems. Try one from our Cell Biology course, then press Mark.

Work out the surface area, volume or surface area to volume ratio of a cube-shaped cell.

Press Mark to have your answer marked in the Cell Biology course.

Hardy-Weinberg

Question: in a population, 9% of people show a recessive condition. What percentage are carriers?

  1. People with the condition are homozygous recessive, so q² = 0.09.
  2. Take the square root: q = 0.3.
  3. Use p + q = 1: p = 0.7.
  4. Carriers are heterozygous: 2pq = 2 × 0.7 × 0.3 = 0.42, so 42% are carriers.

The trap is starting from q = 0.09. The percentage with the condition is q², never q. Check it with the Hardy-Weinberg Calculator.

The chi-squared test

Question: a cross is expected to give tall and short plants in a 3:1 ratio. Of 400 offspring, 290 are tall and 110 are short. Is the difference from 3:1 significant?

The expected numbers are 300 tall and 100 short. Add up (observed − expected)² ÷ expected for each group.

\chi^2 = \frac{(290 - 300)^2}{300} + \frac{(110 - 100)^2}{100} = 0.33 + 1.00 = 1.33

There are two groups, so 1 degree of freedom, and the critical value at p = 0.05 is 3.84. Since 1.33 is less than 3.84, accept the null hypothesis: the difference is due to chance, and the results fit a 3:1 ratio. Always finish with that sentence in context, because the number alone doesn't earn the final mark. Model crosses in the Genetics Cross Lab, then try this question from our Genetics and DNA course.

Type the chance that a child of a random cross has a given trait.

Press Mark to have your answer marked in the Genetics & DNA course.

A-level Biology past papers

The boards publish past papers, mark schemes and examiners' reports free: AQA, OCR and Edexcel. The newest papers are held back for a while: Pearson keeps them for teachers so that at least one can be used as a mock, and releases most once they are nine months old.

Use mark schemes to learn the wording examiners reward, and the examiners' reports to see where students lost marks. When you give a past paper a grade, avoid boundaries from 2020 to 2022, when grading was unusually generous.

Common A-level Biology mistakes

  • "Energy is produced" in respiration. Energy is released, and it is used to make ATP. Energy is never produced.
  • "The enzyme is killed". Enzymes aren't alive. Say the active site changes shape so the enzyme is denatured.
  • Describing when asked to explain. "Describe" wants what the data shows, with numbers. "Explain" wants the biological reason.
  • Ignoring the data. If a question gives a graph or table, quote values from it. General answers miss the marks.
  • Unit slips. Convert µm, mm and cm before you calculate, and give units with every answer.
  • Rounding too early in multi-step calculations. Keep full values until the last line.

Use a daily paper as a short timed mock

Year 13 mocks run through the winter, and a full biology paper takes 2 hours or more. On the days you don't have that, sit today's Year 1 or Year 2 paper instead. For dates and whether mocks count, see the A-level mocks guide.

  1. Clear the desk, with a calculator and ruler, and start the timer.
  2. Answer every part, even if it's only the first step.
  3. Mark it, then read the mark scheme wording for every part where you dropped marks.
  4. Note the topic of each lost mark and revise the biggest one before tomorrow's paper.

Common questions

How many papers are there in A-level Biology?
Three, with every board. AQA's are 2 hours each, OCR's are 2 hours 15 minutes, 2 hours 15 minutes and 1 hour 30 minutes, and Edexcel's depend on whether you take Biology A or Biology B.
When is A-level Biology Paper 1 in 2027?
Monday 7 June 2027 in the afternoon, for AQA, OCR and Edexcel. Paper 2 is on Monday 14 June and Paper 3 on Friday 18 June.
What is the pass mark for A-level Biology?
There is no fixed pass mark. In June 2026 an E took 63 out of 260 with AQA, 72 out of 270 on OCR Biology A and 94 out of 300 on Edexcel Biology A.
Is there an essay in AQA A-level Biology?
Yes. Paper 3 ends with a 25 mark essay, chosen from two titles.
Does the Practical Endorsement affect my grade?
No. It is reported separately from your A to E grade, but you still need to complete at least 12 practical activities for it.
Are these real past papers?
No. Every Uni-Sine paper is original and written in the style of the exam boards, with a new one each day.

Revision cards

Test yourself, then flip each card to check.

Where to go next

  • Solve it

    Hardy-Weinberg Calculator

    Find allele and genotype frequencies in a population

    Open the tool
  • Learn it

    Genetics & DNA

    From Mendel to CRISPR: how traits are inherited and genomes are edited

    Start the course
  • Test it

    A-level Biology: Year 2

    A new exam-style paper every day, with instant marking and a mark scheme

    Start today's paper
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